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KMID : 0379119970250010026
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Korean Journal of Mycology
1997 Volume.25 No. 1 p.26 ~ p.29
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A Positive Selection for Pyrimidine Auxotrophic Mutants from Basidiospores of Pleurotus sajor - caju Using 5´-Fluoro-orotic acid
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박수철/Park, Soo Chul
류진창/권석태/김범기/유영복/정미정/Ryu, Jin Chang/Kwon, Suk Tae/Kim, Beom Gi/Yoo, Young Bok/Jeong, Mi Jeong
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Abstract
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Pyrimidine auxotrophic basidiospores of Pleurotus sajor-caju were selected using positive selection method. Wild type basidiospores could not grow on minimal medium containing the pyrimidine analog 5´-fiuoro-orotic acid (5´-FOA) whereas pyrimidine auxotrophs grew normally, After treatment of basidiospores with ultraviolet light, a total of 13 pyrimidine auxotrophic basidiospores were isolated among 24 5´-FOA resistant mutants. These mutants require the pyrimidine such as uracil, cytosine, thymine. Mating type group and growth rate of their mutants were determined.
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